IL12A ELISA Kit (Human) : 96 Wells (OKEH00096)

Catalog No: OKEH00096

Size:96T

Human IL12A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IL12A ELISA Kit (Human) (OKEH00096) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL12A captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.2% (n = 20)
  Mean Inter-assay CV%: < 10.5% (n = 20)
• ELISA Kit Component:
  • IL12A Microplate: 96 Wells (12 x 8 Well strips)
  • IL12A Lyophilized Standard: 2
  • 100X Biotinylated IL12A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 15 pg/mL
• Specificity: Natural and recombinant Human Interleukin-12 subunit alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL12A
• Gene Full Name: interleukin 12A
• Alias Symbols: CLMF;CLMF p35;cytotoxic lymphocyte maturation factor 1, p35;cytotoxic lymphocyte maturation factor 35 kDa subunit;IL-12 subunit p35;IL-12, subunit p35;IL-12A;IL35 subunit;interleukin 12, p35;interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35);interleukin-12 alpha chain;interleukin-12 subunit alpha;NF cell stimulatory factor chain 1;NFSK;NK cell stimulatory factor chain 1;NKSF1;P35.
• NCBI Gene Id: 3592
• Protein Name: P29459
• Description of Target: Interleukin-12 subunit alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P29459
• Protein Accession #: NP_000873
• Nucleotide Accession #: NM_000882
• Protein Size (# AA): 219
• Molecular Weight: 25 kDa