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Catalog No: OKAA00038-96W
Size:96 Wells
Price: $525.00
SKU
OKAA00038-96W
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IL-12/23(P40) ELISA Kit (Mouse) : 96 Wells (OKAA00038-96W)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit ReproducibilityThe coefficient of variation of both intra-assay and inter-assay were less than 10%.
ELISA Kit Component
Components
2 vials mouse IL-12/IL-23p40 Standard lyophilized, 1000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-mouse IL-12/IL-23p40 antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials mouse IL-12p70 Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-mouse IL-12p70 antibody
Additional InformationSample Type: cell culture supernates, serum, and plasma.
::Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with antibody to mouse IL-12/IL-23p40 (8x12)
2. 2 vials mouse IL-12/IL-23p40 Standard lyophilized, 1000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-mouse IL-12/IL-23p40 antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Typecell culture, supernates, serum, and plasma
Sensitivity4 pg/ml
Predicted Homology Based on Immunogen SequenceMouse
SpecificityThis assay recognizes both natural and recombinant mouse IL-10. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IL-10
Recombinant mouse: IL-1a, IL-1b, IL-2 , IL-4
Recombinant porcine: IL-10
Assay InfoAssay Method: Quantitative Sandwich ELISA
Gene SymbolIL12B
Gene Full Nameinterleukin 12B
Alias SymbolsCLMF, NKSF, CLMF2, IMD28, IMD29, NKSF2, IL-12B
NCBI Gene Id3593
Protein NameInterleukin-12 subunit beta
Description of TargetThis gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Uniprot IDP29460
Protein Accession #NP_002178
Nucleotide Accession #NM_002187
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