Catalog No: OKCD08699
Size:96WELLS
Price: $850.00
SKU
OKCD08699
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Datasheets/ManualsPrintable datasheet for IL11RA1 ELISA Kit (Rat) (OKCD08699)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 11 Receptor Alpha (IL11Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 11 Receptor Alpha (IL11Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 11 Receptor Alpha (IL11Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 11 Receptor Alpha (IL11Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 11 Receptor Alpha (IL11Ra) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 11 Receptor Alpha (IL11Ra) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
IL11RA1 Microplate96 Wells (12 x 8 Well strips)
IL11RA1 Lyophilized Standard2
100X IL11RA1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.064ng/mL
Gene SymbolIl11ra1
Gene Full Nameinterleukin 11 receptor subunit alpha 1
Alias SymbolsIL-11 receptor subunit alpha;IL-11R subunit alpha;IL-11RA;IL-11R-alpha;interleukin 11 receptor, alpha chain 1;interleukin-11 receptor alpha chain;interleukin-11 receptor subunit alpha.
NCBI Gene Id245983
Protein NameInterleukin-11 receptor subunit alpha
Description of TargetReceptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells.
Uniprot IDQ99MF4
Protein Accession #NP_620816.1
Nucleotide Accession #NM_139116.1
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