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Catalog No: OKDD00719 (Formerly GWB-KBBUO1)
Size:96 Wells
Price: $725.00
SKU
OKDD00719
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Datasheets/ManualsPrintable datasheet for IL10Rb ELISA Kit (Rat) (OKDD00719)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology IL10Rb ELISA Kit (Rat) (OKDD00719) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IL10Rb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL10Rb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL10Rb captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-IL10Rb Microplate96 Wells (12 x 8 Well Strips)
IL10Rb Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X IL10Rb Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates
Sensitivity<0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of IL10Rb. No significant cross-reactivity or interference between IL10Rb and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolIl10rb
Gene Full Nameinterleukin 10 receptor subunit beta
Alias Symbolsinterleukin 10 receptor, beta;interleukin-10 receptor subunit beta;RGD1560373.
NCBI Gene Id304091
Protein NameInterleukin 10 receptor subunit beta
Uniprot IDD3ZM36
Protein Accession #NP_001100581.1
Nucleotide Accession #NM_001107111.1
Protein Size (# AA)351
Molecular Weight40 kDa
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