Catalog No: OKDD00718 (Formerly GWB-KBBVR7)
Size:96 Wells
Price: $725.00
SKU
OKDD00718
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for IL10Ra ELISA Kit (Rat) (OKDD00718) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology IL10Ra ELISA Kit (Rat) (OKDD00718) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IL10Ra has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL10Ra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL10Ra captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV<10% Mean Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates | ||||||||||||||||||||||
Sensitivity | <0.063 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of IL10Ra. No significant cross-reactivity or interference between IL10Ra and analogues was observed. | ||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | Il10ra |
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Gene Full Name | interleukin 10 receptor subunit alpha |
Alias Symbols | interleukin 10 receptor, alpha;interleukin-10 receptor subunit alpha. |
NCBI Gene Id | 117539 |
Protein Name | Interleukin 10 receptor subunit alpha |
Description of Target | Alpha chain of receptor for interleukin 10 that may play a role as a central regulator of inflammation [rgd, feb 2006] |
Uniprot ID | G3V830 |
Protein Accession # | NP_476541.2 |
Nucleotide Accession # | NM_057193.2 |
Protein Size (# AA) | 569 |
Molecular Weight | 63 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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