Catalog No: OKDD00718 (Formerly GWB-KBBVR7)
Size:96 Wells
Price: $725.00
SKU
OKDD00718
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Datasheets/ManualsPrintable datasheet for IL10Ra ELISA Kit (Rat) (OKDD00718)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology IL10Ra ELISA Kit (Rat) (OKDD00718) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IL10Ra has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL10Ra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL10Ra captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-IL10Ra Microplate96 Wells (12 x 8 Well Strips)
IL10Ra Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X IL10Ra Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates
Sensitivity<0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of IL10Ra. No significant cross-reactivity or interference between IL10Ra and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolIl10ra
Gene Full Nameinterleukin 10 receptor subunit alpha
Alias Symbolsinterleukin 10 receptor, alpha;interleukin-10 receptor subunit alpha.
NCBI Gene Id117539
Protein NameInterleukin 10 receptor subunit alpha
Description of TargetAlpha chain of receptor for interleukin 10 that may play a role as a central regulator of inflammation [rgd, feb 2006]
Uniprot IDG3V830
Protein Accession #NP_476541.2
Nucleotide Accession #NM_057193.2
Protein Size (# AA)569
Molecular Weight63 kDa
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