Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKEH04509
Size:96T
Price: $500.00
SKU
OKEH04509
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Il10 ELISA Kit (Rat) (OKEH04509) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Il10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il10 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Il10 Microplate96 Wells (12 x 8 Well Strips)
Il10 Lyophilized Standard2
Sample Diluent1 x 20 mL
Il10 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity15.67 pg/mL
SpecificityNatural and recombinant Rat Interleukin-10
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIl10
Gene Full Nameinterleukin 10
Alias SymbolsCSIF;cytokine synthesis inhibitory factor;If2a;IL-10;IL10X;interferon 2a;interleukin-10.
NCBI Gene Id25325
Protein NameP29456
Description of TargetInterleukin-10
Uniprot IDhttps://www.uniprot.org/uniprot/P29456
Protein Accession #NP_036986.2
Nucleotide Accession #NM_012854.2
Protein Size (# AA)178
Molecular Weight20 KDa
Write Your Own Review
You're reviewing:IL10 ELISA Kit (Rat) (OKEH04509)
Your Rating
We found other products you might like!