IL10 ELISA Kit (Human) (OKBB00193)

Catalog No: OKBB00193 (Formerly GWB-ZZD005)

Size:96 Tests

Human IL10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-N178) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-10 amount of sample captured in plate.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	25	112	280	34	184	312
  St. Dev.	1.35	5.38	8.68	2.55	14.54	20.9
  %CV	5.4	4.80	3.1	7.5	7.90	6.69

• ELISA Kit Component:
  • Lyophilized recombinant human IL10 standard: 10 ng/tube x 2
  • Anti-human IL10 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human IL10 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 7.8pg/ml-500pg/ml (body fluids, tissue lysates or cell culture supernates)
  3.4pg/ml-250pg/ml (human sera, plasma).
  Principle: Aviva's human IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IL-10 specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-10 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don't let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don't reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <0.5 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Alamartine, E.; Berthoux, P.; Mariat, C.; Cambazard, F.; Berthoux, F. Interleukin-10 promoter polymorphisms and susceptibility to skin
  squamous cell carcinoma after renal transplantation. J. Invest. Derm. 120: 99-103, 2003.
  2. Grove, J.; Daly, A. K.; Bassendine, M. F.; Gilvarry, E.; Day, C. P. Interleukin 10 promoter region polymorphisms and susceptibility to
  advanced alcoholic liver disease. Gut 46: 540-545, 2000.
  3. Eskdale, J.; Kube, D.; Tesch, H.; Gallagher, G. Mapping of the human IL10 gene and further characterization of the 5-prime flanking
  sequence. Immunogenetics 46: 120-128, 1997.
  4. Gesser, B.; Leffers, H.; Jinquan, T.; Vestergaard, C.; Kirstein, N.; Sindet-Pedersen, S.; Jensen, S. L.; Thestrup-Pedersen, K.; Larsen, C. G.
  Identification of functional domains on human interleukin 10. Proc. Nat. Acad. Sci. 94: 14620-14625, 1997.
  5. Asadullah, K.; Eskdale, J.; Wiese, A.; Gallagher, G.; Friedrich, M.; Sterry, W. Interleukin-10 promoter polymorphism in psoriasis. J. Invest.
  Derm. 116: 975-978, 2001.
  6. Opdal, S. H.; Opstad, A.; Vege, A.; Rognum, T. O. IL-10 gene polymorphisms are associated with infectious cause of sudden infant death.
  Hum. Immun. 64: 1183-1189, 2003.
  7. Kim, J. M.; Brannan, C. I.; Copeland, N. G.; Jenkins, N. A.; Khan, T. A.; Moore, K. W. Structure of the mouse Il-10 gene and chromosomal
  localization of the mouse and human genes. J. Immun. 148: 3618-3623, 1992.
• Specificity: Natural and recombinant human IL-10
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: S19-N178
• Description: For quantitative detection of human IL-10 in cell culture supernatants, serum and plasma( heparin, EDTA, citrate).

Target Info

• Gene Symbol: IL10
• Gene Full Name: interleukin 10
• Alias Symbols: CSIF, Cytokine synthesis inhibitory factor, GVHDS, IL 10, IL-10, IL10, IL10_HUMAN, IL10A, Interleukin 10, Interleukin-10, Interleukin10, MGC126450, MGC126451, T cell growth inhibitory factor, TGIF
• NCBI Gene Id: 3586
• Protein Name: Interleukin-10
• Description of Target: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
• Uniprot ID: P22301
• Protein Accession #: NP_000563.1
• Nucleotide Accession #: NM_000572.2