Catalog No: OKCA01038
Size:96 Wells
Price: $857.00
SKU
OKCA01038
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat10293-106
1:29385-97
1:49789-101
1:89689-100
ELISA Kit PrincipleAviva Systems Biology IL10 ELISA Kit (Cat) (OKCA01038) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL10 captured in the well.
ELISA Kit Range31.25 - 2,000 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)10094-105
EDTA Plasma (n=4)9593-100
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level OKCA01038 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01038 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
IL10 Microplate96 Wells (12 x 8 Well strips)
IL10 Lyophilized Standard2 x 2 ng
100X Biotinylated IL10 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity7.81 pg/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolIL10
Gene Full NameInterleukin-10
Alias SymbolsCSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
NCBI Gene Id493683
Protein NameInterleukin-10
Description of TargetInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Uniprot IDP55029
Protein Accession #NP_001009209.1
Nucleotide Accession #NM_001009209.1
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