IL10 Chemi-Luminescent ELISA Kit (Human) (OKCD03614)

Catalog No: OKCD03614

Size:96WELLS

Human IL10 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	97-105%	97-105%	90-99%
  EDTA Plasma (n=5)	82-93%	87-94%	89-97%	86-97%
  Heparin Plasma (n=5)	95-103%	86-95%	89-97%	98-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 10 (IL10) level in the sample or standard.
• ELISA Kit Range: 0.69-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-99	96
  EDTA Plasma (n=5)	79-104	82
  Heparin Plasma (n=5)	82-98	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL10 Microplate: 96 Wells (12 x 8 Well strips)
  • IL10 Lyophilized Standard: 2
  • 100X IL10 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.69 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL10
• Gene Full Name: interleukin 10
• Alias Symbols: CSIF;cytokine synthesis inhibitory factor;GVHDS;IL-10;IL10A;interleukin-10;T-cell growth inhibitory factor;TGIF.
• NCBI Gene Id: 3586
• Protein Name: Interleukin-10
• Description of Target: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
• Uniprot ID: P22301
• Protein Accession #: NP_000563
• Nucleotide Accession #: NM_000572