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IL10 Antibody (OASA04721)

1 mg
$1,010.00
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Predicted Species Reactivity:
Human
Product Format:
Purified IgG - liquid
Clonality:
Monoclonal
Clone:
JES3-12G8
Isotype:
IgG2a
Host:
Rat
Conjugation:
AF: Azide Free
Application:
WB
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: None Present
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline
Reconstitution and Storage:
Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Symbol:
IL10
NCBI Gene Id:
3586
Protein Name:
Interleukin-10
Swissprot Id:
P22301
Protein Accession #:
NP_000563.1
Alias Symbols:
CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, IL10
Replacement Item:
This antibody may replace item sc-1783 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI HUMAN INTERLEUKIN-10:Azide Free
Protein Size (# AA):
178
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL10.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL10.
Immunogen:
The immunogen for anti-IL10 antibody: human IL-10 expressed by COS cells
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Printable datasheet for anti-IL10 antibody - OASA04721
Specificity:
IL-10
Application Info:
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul Method sheets are available on request
Application Data:
Application #1: Western blot analysis of human IL-10 recombinant protein probed with Rat anti Human Interleukin-10 followed by F(ab')2 Rabbit anti Rat IgG:HRP
Target Reference:
1. Yssel, H. et al. (1992) IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells. J. Immunol. 149:2378-2384.
2. Burdin, N. et al. (1993) Epstein-Barr virus transformation induces B lymphocytes to produce human Interleukin-10. J. Exp. Med. 177:295-304.
3. Gomes, J. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response. Infect. Immun. 71:1185-1193.
4. Abrams, J. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.

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