- Predicted Species Reactivity:
- Product Format:
- Purified IgG - liquid
- Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
- Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- NCBI Gene Id:
- Protein Name:
- Alias Symbols:
- CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, IL10
- Replacement Item:
- This antibody may replace item sc-1783 from Santa Cruz Biotechnology.
- Description of Target:
- RAT ANTI HUMAN INTERLEUKIN-10
- Protein Size (# AA):
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express IL10.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express IL10.
- The immunogen for anti-IL10 antibody: human IL-10 expressed by COS cells
- Predicted Homology Based on Immunogen Sequence:
- Printable datasheet for anti-IL10 antibody - OASA04720
- Application Info:
- Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections.
Enzyme pretreatment using pronase or
- Application Data:
- Application #1: Western blot analysis of human IL-10 recombinant protein probed with Rat anti Human Interleukin-10 followed by F(ab')2 Rabbit anti Rat IgG:HRP
- Target Reference:
- 1. Yssel, H. et al. (1992) IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells. J. Immunol. 149:2378-2384.
2. Burdin, N. et al. (1993) Epstein-Barr virus transformation induces B lymphocytes to produce human Interleukin-10. J. Exp. Med. 177:295-304.
3. Gomes, J. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response. Infect. Immun. 71:1185-1193.
4. Abrams, J. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.