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IL10 Antibody - Biotin Conjugated (OASA00345)

0.25 mg
$780.00
In Stock
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Gene Symbol:
IL10
NCBI Gene Id:
281246
Protein Name:
Interleukin-10
Swissprot Id:
P43480
Protein Accession #:
NP_776513.1
Replacement Item:
This antibody may replace item sc-1783 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI BOVINE INTERLEUKIN-10:Biotin
Protein Size (# AA):
178
Host:
Mouse
Clonality:
Monoclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL10.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL10.
Immunogen:
The immunogen for anti-IL10 antibody: plasmid cDNA encoding bovine IL-10
Predicted Species Reactivity:
Cow
Predicted Homology Based on Immunogen Sequence:
Cow
Product Format:
Purified IgG conjugated to Biotin - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-IL10 antibody - OASA00345
Specificity:
IL-10
Clone:
CC320
Conjugation:
Biotin
Isotype:
IgG1
Application Info:
ELISA : This biotin conjugate may be used as detection reagent in a sandwich ELISA assay for bovine IL-10 with OASA00344 as capture reagent.
Additional Information:
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse sp2/0 myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Soduim Azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline
Target Reference:
1. Kwong, L. S. et al. (2002) Development of an ELISA for bovine IL-10. Vet. Immunol. Immunopathol. 85: 213-223.
2. Buza, J.J. et al. (2004) Neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium avium subsp.paratuberculosis in experimentally infected cattle with paratuberculosis. Infect. Immun. 72(4): 2425-8.
3. Scandurra, G.M. et al. (2010) Assessment of live candidate vaccines for paratuberculosis in animal models and

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