IL-8 ELISA Kit (Human) (OKGD00025)

Catalog No: OKGD00025

Size:96 Wells

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 2 hours
• ELISA Kit Principle: Aviva Systems Biology’s IL-8 ELISA Kit (Human) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL-8 has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A Biotin conjugated detector antibody specific for IL- 8 is added, incubated and washed. Next it is incubated with horseradish peroxidase (HRP)-Streptavidin, followed by wash. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL-8 captured in well.
• ELISA Kit Range: 31.25-2000pg/ml
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 10%
  Mean Inter-assay CV%: <= 10%
• ELISA Kit Component:
  • Anti-IL-8 Microplate: 1 x 96 wells plate
  • IL-8 Lyophilized Standard: 2 x vials (4000pg/ml/vial)
  • Human IL-8 Biotin Detection Antibody: 1 x 30 uL
  • Assay Diluent A: 1 x 50 mL
  • HRP-Streptavidin: 1 x 30 uL
  • 20X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 12 mL
  • Stop Solution: 1 x 12 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: <5pg/ml
• Concentration: 4000pg/ml
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CXCL8
• Gene Full Name: C-X-C motif chemokine ligand 8
• Alias Symbols: IL8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, SCYB8
• NCBI Gene Id: 3576
• Protein Name: Interleukin-8
• Description of Target: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
• Uniprot ID: P10145
• Protein Accession #: NP_000575.1
• Nucleotide Accession #: NM_000584.3