IL 6 Protein (OPPA00650)

Catalog No: OPPA00650 (Formerly GWB-CC5D17)

Size:2UG

Product Info

• Predicted Species Reactivity: Rat
• Product Format: The protein was lyophilized without any additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Interleukin-6 in 100mM acetic acid to 1.0 mg/mL and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions.
  Biological Activity: The ED50= 0.03-0.1 ng/mL. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. A concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
  Product Introduction: Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
  Product Description: Interleukin-6 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton. The IL6 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL6 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Met - Phe - Pro - Thr - Ser.

Target Info

• Gene Symbol: IL6
• Alias Symbols: ILg6, Ifnb2
• NCBI Gene Id: 24498
• Protein Name: Interleukin-6
• Description of Target: Recombinant Rat Interleukin-6
• Uniprot ID: P20607
• Protein Accession #: NP_036721.1
• Protein Size (# AA): Recombinant