IL-6 High Sensitivity ELISA Kit (Human) (OKDB00046)

Catalog No: OKDB00046

Human IL-6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Linearity: 4 human pooled serums samples with different levels of IL-6 were analysed at different serial two fold dilutions (1:2 to 1:8) with two replicates each.
• ELISA Kit Principle: Aviva Systems Biology IL-6 High Sensitivity ELISA Kit (Human) (OKDB00046) is designed for the highly sensitive in-vitro quantitative measurement of recombinant or natural IL-2 in supernatants, buffered solutions or serum and plasma samples and other body fluids. The kit is based on standard sandwich enzyme-linked immuno-sorbent assay methods along with an additional detection process employing a polymerization step to amplify the number of detector bound HRP molecules. A monoclonal antibody specific for IL-6 has been pre-coated into wells of a 96-well plate. Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for IL-6 is added, incubated and followed by washing. Streptavidin-HRP conjugate is then added, incubated and unbound conjugate is washed away. Amplification is achieved by adding a Biotin-Tyramine reagent, incubating and washing, followed by a second incubation with Streptavidin-HRP conjugate. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL-6 captured in well.
• ELISA Kit Range: 1.56 - 50 pg/mL
• ELISA Kit Recovery: The spike recovery was evaluated by spiking 3 concentrations of IL-6 into human serum in 2 separate experiments. Recoveries ranged from 107% to 123% with an overall mean recovery of 115%.
• ELISA Kit Reproducibility: Four sera and one cell culture medium samples with various concentrations of IL-6 were tested for repeatability and reproducibility. Each assay was carried out with 3 replicates of each sample. Five independent assays were performed. The intra-assay and inter-assay coefficient of variation has been calculated to be 4.4% and 9.1% respectively.
• ELISA Kit Component:

  Reagents	1 x 48 Well Kit	1 x 96 Well Kit	2 x 96 Well Kit
  IL-6 Well Strip Microplate	6 x 8 Wells	12 x 8 Wells	24 x 8 Wells
  IL-4 Standard	1 Vial	2 Vials	4 Vials
  10X Standard Diluent (Buffer)	1 x 25 mL	1 x 25 mL	1 x 25 mL
  27.5X Biotinylated anti-IL-4 Detector Antibody	1 x 400 uL	1 x 400 uL	2 x 400 uL
  Biotinylated Detector Antibody Diluent	1 x 7 mL	1 x 7 mL	1 x 13 mL
  Streptavidin-HRP	1 x 5 uL	2 x 5 uL	4 x 5 uL
  Amplification Diluent	1 x 25 mL	1 x 25 mL	1 x 25 mL
  100X Amplifier	1 x 200 uL	1 x 200 uL	2 x 200 uL
  HRP Diluent	1 x 25 mL	1 x 25 mL	2 x 25 mL
  200X Wash Buffer	1 x 10 mL	1 x 10 mL	2 x 10 mL
  TMB Substrate	1 x 11 mL	1 x 11 mL	2 x 24 mL
  H2SO4 Stop Reagent	1 x 11 mL	1 x 11 mL	2 x 11 mL

• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma and Other body fluids
• Sensitivity: < 0.81 pg/mL
• Specificity: The assay recognizes both natural and recombinant human IL-6. To define the specificity of this ELISA several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1a, IL-b, IL-10, IL-12, IFNgamma, IL-4, TNFa, IL-8 and IL-13).
• Publications:

  In Systemic Sclerosis, a Unique Long Non Coding RNA Regulates Genes and Pathways Involved in the Three Main Features of the Disease (Vasculopathy, Fibrosis and Autoimmunity) and in Carcinogenesis. J Clin Med. 8, (2019). 30866419

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for IL-6 High Sensitivity ELISA Kit (Human) (OKDB00046) on CiteAb

Target Info

• Gene Symbol: IL-6
• Gene Full Name: interleukin 6
• Alias Symbols: CDF, HGF, HSF, BSF2, IL-6, BSF-2, IFNB2, IFN-beta-2
• NCBI Gene Id: 3569
• Protein Name: interleukin-6 isoform 1 precursor
• Description of Target: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
• Uniprot ID: P05231
• Protein Accession #: NP_000591.1
• Nucleotide Accession #: NM_000600.4
• Protein Size (# AA): 212
• Molecular Weight: 24 kDa