IL-6 ELISA Kit (Rat) (OKBB00191)

Catalog No: OKBB00191 (Formerly GWB-ZZD100)

Size:96 Tests

Rat IL-6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's rat IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, F25-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat IL-6 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	150	392	1635	176	415	1474
  St. Dev.	13.65	34.89	91.56	16.72	38.18	110.55
  %CV	9.1	8.90	5.6	9.5	9.2	7.5

• ELISA Kit Component:
  • Lyophilized recombinant human IL-5 standard: 10 ng/tube x 2
  • Anti-human IL-5 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human IL-5 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 62.5pg/ml-4000pg/ml
  Principle: Aviva’s rat IL-6 ELISA Kit was based on standard sandwich enzyme-linked
  immune-sorbent assay technology. Rat IL-6 specific-specific polyclonal
  antibodies were precoated onto 96-well plates. The rat specific detection
  polyclonal antibodies were biotinylated. The test samples and biotinylated
  detection antibodies were added to the wells subsequently and then followed
  by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was
  added and unbound conjugates were washed away with PBS or TBS buffer.
  HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was
  catalyzed by HRP to produce a blue color product that changed into yellow
  after adding acidic stop solution. The density of yellow is proportional to the
  rat IL-6 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene.1 Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.2 IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption.3 IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH).4 Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <5 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Ferguson-Smith AC, Chen YF, Newman MS, May LT, Sehgal PB, Ruddle FH (April 1988). "Regional localization of
  the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome 7p15-p21".
  Genomics 2 (3): 203–8.
  2. Fernandez-Real, J.-M., Broch, M., Vendrell, J., Richart, C., Ricart, W. Interleukin-6 gene polymorphism and lipid
  abnormalities in healthy subjects. J. Clin. Endocr. Metab. 85: 1334-1339, 2000.
  3. Ferrari, S. L., Ahn-Luong, L., Garnero, P., Humphries, S. E., Greenspan, S. L. Two promoter polymorphisms
  regulating interleukin-6 gene expression are associated with circulating levels of C-reactive protein and markers of
  bone resorption in postmenopausal women. J. Clin. Endocr. Metab. 88: 255-259, 2003.
  4. Venihaki, M., Dikkes, P., Carrigan, A., Karalis, K. P. Corticotropin-releasing hormone regulates IL-6 expression during
  inflammation. J. Clin. Invest. 108: 1159-1166, 2001.
• Specificity: Natural and recombinant rat IL-6
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: F25-T211
• Description: For quantitative detection of rat IL-6 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).

Target Info

• Gene Symbol: IL6
• Gene Full Name: interleukin 6
• Alias Symbols: B cell differentiation factor, B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating fact, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFN-beta-2, IFNB2, IL 6, IL-6, IL6, IL6 protein, IL6_HUMAN, Interferon beta 2, Interferon beta-2, Interleukin 6, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, Interleukin6
• NCBI Gene Id: 24498
• Protein Name: Interleukin-6
• Description of Target: Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene.1 Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.2 IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption.3 IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH).4 Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130.5 The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
• Uniprot ID: P20607
• Protein Accession #: NP_036721.1
• Nucleotide Accession #: NM_012589.2