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IL-6 ELISA Kit (Human) (OKBB00189)

96 Tests
$335.00
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Application:
ELISA
Gene Symbol:
IL-6
Official Gene Full Name:
interleukin 6
NCBI Gene Id:
3569
Alias Symbols:
Interleukin-6, Interleukin 6, IL 6, IFNB2
Description of Target:
The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells.1 Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency.2 IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption.3 It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.4 Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline.5 BSF-2 is a novel interleukin consisting of 184 amino acids.6 The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
Protein Name:
Interleukin-6
Swissprot Id:
P05231
Protein Accession #:
NP_000591.1
Nucleotide Accession #:
NM_000600.4
Predicted Species Reactivity:
Human
Sample Type:
cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sensitivity:
< 0.3 pg/ml
Kit Range:
4.69 pg/ml-300 pg/ml
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)16.39817918.299185
St. Dev.0.82.34.213.65.7
%CV4.902.342.345.493.633.08
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology IL-6 ELISA Kit (Human) (OKBB00189) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for IL-6 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (E.coli, P29-M212) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL-6 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human IL-6 captured in well.
Kit Component:
ComponentAmount
Lyophilized recombinant human IL-5 standard10 ng/tube x 2
Anti-human IL-5 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- human IL-5 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL-6.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL-6.
Predicted Homology Based on Immunogen Sequence:
No detectable cross-reactivity with any other cytokine.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Target Reference:
1. Bowcock, A. M.; Kidd, J. R.; Lathrop, G. M.; Daneshvar, L.; May, L. T.; Ray, A.; Sehgal, P. B.; Kidd, K. K.; Cavalli-Sforza, L. L. The human
'interferon-beta-2/hepatocyte stimulating factor/interleukin-6' gene: DNA polymorphism studies and localization to chromosome 7p21.
Genomics 3: 8-16, 1988.
2. Ota, N.; Nakajima, T.; Nakazawa, I.; Suzuki, T.; Hosoi, T.; Orimo, H.; Inoue, S.; Shirai, Y.; Emi, M. A nucleotide variant in the promoter region
of the interleukin-6 gene associated with decreased bone mineral density. J. Hum. Genet. 46: 267-272, 2001. 3. Chung, H. W.; Seo, J.-S.; Hur, S. E.; Kim, H. L.; Kim, J. Y.; Jung, J. H.; Kim, L. H.; Park, B. L.; Shin, H. D. Association of interleukin-6
promoter variant with bone mineral density in pre-menopausal women. J. Hum. Genet. 48: 243-248, 2003.
4. Ferrari, S. L.; Ahn-Luong, L.; Garnero, P.; Humphries, S. E.; Greenspan, S. L. Two promoter polymorphisms regulating interleukin-6 gene
expression are associated with circulating levels of C-reactive protein and markers of bone resorption in postmenopausal women. J. Clin.
Endocr. Metab. 88: 255-259, 2003.
5. Kiecolt-Glaser, J. K.; Preacher, K. J.; MacCullum, R. C.; Atkinson, C.; Malarkey, W. B.; Glaser, R. Chronic stress and age-related increases
in the proinflammatory cytokine IL-6. Proc. Nat. Acad. Sci. 100: 9090-9095, 2003.
6. Hirano, T.; Yasukawa, K.; Harada, H.; Taga, T.; Watanabe, Y.; Matsuda, T.; Kashiwamura, S.; Nakajima, K.; Koyama, K.; Iwamatsu, A.;
Tsunasawa, S.; Sakiyama, F.; Matsui, H.; Takahara, Y.; Taniguchi, T.; Kishimoto, T. Complementary DNA for a novel human interleukin
(BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature 324: 73-76, 1986.
Specificity:
There is no detectable cross-reactivity with other relevant proteins.
Publications:

Ota, H. et al. Pathological mechanism of musculoskeletal manifestations associated with CRPS type II: An animal study. Pain. 155, 1976-85 (2014). ELISA, Human 25016218

Selfe, J. et al. The effect of three different (-135°C) whole body cryotherapy exposure durations on elite rugby league players. PLoS One 9, e86420 (2014). ELISA, Human 24489726

Additional Information:
Range: 4.69pg/ml-300pg/ml
:::
Principle: Aviva’s human IL-6 ELISA Kit was based on standard sandwich
enzyme-linked immune-sorbent assay technology. Human IL-6 specific-specific
monoclonal antibodies were precoated onto 96-well plates. The human specific
detection polyclonal antibodies were biotinylated. The test samples and
biotinylated detection antibodies were added to the wells subsequently and
then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase
Complex was added and unbound conjugates were washed away with PBS or
TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction.
TMB was catalyzed by HRP to produce a blue color product that changed into
yellow after adding acidic stop solution. The density of yellow is proportional to
the human IL-6 amount of sample captured in plate
:::
Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells.1 Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency.2 IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption.3 It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.4 Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline.5 BSF-2 is a novel interleukin consisting of 184 amino acids.6 The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

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