Catalog No: OPPA02015 (Formerly GWB-D4B59B)
Size:2UG
Price: $33.00
SKU
OPPA02015
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OPPA02015 |
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Predicted Species Reactivity | Mouse |
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Product Format | Lyophilized from a concentrated (1mg/ml) solution containing 20mM Sodium Phosphate and 50mM NaCl pH-7.5. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Host | E. Coli |
Additional Information | Solubility: It is recommended to reconstitute the lyophilized Mouse Interleikin-5 in sterile 18M Omega -cm H2O at a concentration of 100 ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of TF-1 cells was found to be 1.7ng/ml. |
:: | Product Introduction: The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. Product Description: Interleukin-5 Mouse Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 2 x 113 amino acids forming a disulfide linked homodimer and having a molecular mass of 26.2 kDa.The Mouse IL-5 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized Mouse Interleukin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Mouse IL5 should be stored at 4C between 2-7 days and for future use below -18C. Please avoid freeze thaw cycles. |
Purity | Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Peptide Sequence | MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG. |
Gene Symbol | IL5 |
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Alias Symbols | Il, Il-5 |
NCBI Gene Id | 16191 |
Protein Name | Interleukin-5 |
Description of Target | Recombinant Mouse Interleukin-5 |
Uniprot ID | P04401 |
Protein Accession # | NP_034688.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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