IL 5 Protein (OPPA00477)

Catalog No: OPPA00477 (Formerly GWB-A4A0A5)

Size:2UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Interleikin-5 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of TF-1 cells was found to be < 0.15ng/ml, corresponding to a Specific Activity of 6 x 10⁶ IU/mg.
  Product Introduction: The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
  Product Description: Interleukin-5 Human Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 26522.84 Dalton.The IL-5 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Interleukin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL5 should be stored at 4C between 2-7 days and for future use below -18C. Please avoid freeze thaw cycles.
• Purity: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Met - Ile - Pro - Thr - Glu.

Target Info

• Gene Symbol: IL5
• Alias Symbols: EDF, TRF, IL-5
• NCBI Gene Id: 3567
• Protein Name: Interleukin-5
• Description of Target: Recombinant Human Interleukin-5
• Uniprot ID: P05113
• Protein Accession #: NP_000870.1
• Protein Size (# AA): Recombinant