IL 4 Protein (OPPA00476)

Catalog No: OPPA00476 (Formerly GWB-DAF66B)

Size:2ug

Product Info

• Product Format: Lyophilized PBS (pH 7.4) from a 0.2 um filtered solution in PBS (pH 7.4)
  Physical Appearance: Sterile filtered white freeze-dried powder
• Host: E. Coli
• Additional Information: Protein Content: Protein quantitation was carried out by two independent methods:
  1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
• Reconstitution and Storage: Reconstitution: It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Storage: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL4 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purification: Purified by proprietary chromatographic techniques.
• Concentration: 1 mg/ml (prior to lyophilization)
• Purity: Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: The ED₅₀ as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5 x 10⁶ IU/mg.
• Peptide Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS.

Target Info

• Gene Symbol: IL4
• Alias Symbols: BSF1, IL-4, BCGF1, BSF-1, BCGF-1
• NCBI Gene Id: 3565
• Protein Name: Interleukin-4
• Description of Target: IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids.
• Uniprot ID: P05112
• Protein Accession #: NP_000580.1
• Protein Size (# AA): 130 aa
• Molecular Weight: 15 kDa