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Catalog No: OKDB00045
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OKDB00045
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IL-4 High Sensitivity ELISA Kit (Human) (OKDB00045)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Duration< 4 Hours
ELISA Kit LinearityThe linear regression of samples versus the expected concentration yielded a quote slope of 0.992.
ELISA Kit PrincipleAviva Systems Biology IL-4 High Sensitivity ELISA Kit (Human) (OKDB00045) is designed for the highly sensitive in-vitro quantitative measurement of recombinant or natural IL-4 in human sera, plasmas, buffered solutions or cell culture media. The kit is based on standard sandwich enzyme-linked immuno-sorbent assay methods along with an additional detection process employing a polymerization step to amplify the number of detector bound HRP molecules. A monoclonal antibody specific for IL-4 has been pre-coated into wells of a 96-wellplate. Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for IL-4 is added, incubated and followed by washing. Streptavidin-HRP conjugate is then added, incubated and unbound conjugate is washed away. Amplification is achieved by adding a Biotin-Tyramine reagent, incubating and washing, followed by a second incubation with Streptavidin-HRP conjugate. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL-4 captured in well.
ELISA Kit Range0.31 - 10 pg/mL
ELISA Kit RecoveryThe recovery in pooled human sera ranged from 61 to 75% with a mean of 69%. In cell culture medium, recovery was 897% and 127%, with a mean of 95%.
ELISA Kit ReproducibilityTwo sera and two cell culture medium samples with various concentrations of IL-4 were tested for repeatability and reproductibility. Each assay was carried out with 8 replicates of each sample. Three independent assays were performed. The intra-assay and inter-assay coefficient of variation has been calculated to be 9.2% and 13.7% respectively
ELISA Kit Component
Reagents1 x 48 Well Kit1 x 96 Well Kit2 x 96 Well Kit
IL-4 Well Strip Microplate6 x 8 Wells12 x 8 Wells24 x 8 Wells
IL-4 Standard1 Vial2 Vials4 Vials
10X Standard Diluent (Buffer)1 x 25 mL1 x 25 mL1 x 25 mL
27.5X Biotinylated anti-IL-4 Detector Antibody1 x 400 uL1 x 400 uL2 x 400 uL
Biotinylated Detector Antibody Diluent1 x 7 mL1 x 7 mL1 x 13 mL
Streptavidin-HRP1 x 5 uL2 x 5 uL4 x 5 uL
Amplification Diluent1 x 25 mL1 x 25 mL1 x 25 mL
100X Amplifier1 x 200 uL1 x 200 uL2 x 200 uL
HRP Diluent1 x 25 mL1 x 25 mL2 x 25 mL
200X Wash Buffer1 x 10 mL1 x 10 mL2 x 10 mL
TMB Substrate1 x 11 mL1 x 11 mL2 x 24 mL
H2SO4 Stop Reagent1 x 11 mL1 x 11 mL2 x 11 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSera, Plasmas, Buffered solutions or Cell culture media
Sensitivity< 0.31 pg/mL
SpecificityTen specificities were tested with concentrations higher than IL-4 curve concentrations. No cross reaction was observed for IL-2, IL-5, IL-6, IL-7, IL-8, IL-12, IL-13, TNF-alpha, IFNgamma and FasL.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL-4
Gene Full Nameinterleukin 4
Alias SymbolsBSF1, IL-4, BCGF1, BSF-1, BCGF-1
NCBI Gene Id3565
Protein Nameinterleukin-4 isoform 1 precursor
Description of TargetThe protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Uniprot IDP05112
Protein Accession #NP_000580.1
Nucleotide Accession #NM_000589.3
Protein Size (# AA)153
Molecular Weight17 kDa
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