IL-4 ELISA Kit (Mouse) (OKGD00024)

Catalog No: OKGD00024

Size:96 Wells

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 2 hours
• ELISA Kit Principle: Aviva Systems Biology’s IL-4 ELISA Kit (Mouse) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL-4 has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A biotin conjugated detector antibody specific for IL-4 is added, incubated and followed by wash. Next it is incubated with horseradish peroxidase(HRP) Streptavidin, followed by wash. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL-4 captured in well.
• ELISA Kit Range: 3.13-200pg/ml 
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 10%
  Mean Inter-assay CV%: <= 10%
• ELISA Kit Component:
  • Anti-IL-4 Microplate: 1 x 96 wells plate
  • IL-4 Lyophilized Standard: 2 x vials (500pg/ml/vial)
  • Mouse IL-4 Biotin Detection Antibody: 1 x 30 uL
  • Assay Diluent A: 1 x 50 mL
  • HRP-Streptavidin: 1 x 30 uL
  • 20X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 12 mL
  • Stop Solution: 1 x 12 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: <0.5pg/ml
• Concentration: 500pg/ml
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL-4
• Gene Full Name: interleukin 4
• Alias Symbols: Il, Il-4, BSF-1
• NCBI Gene Id: 16189
• Protein Name: Interleukin-4
• Description of Target: Participates in at least several B-cell activation processes as well as of other cell types . It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells . It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages . Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
• Uniprot ID: P07750
• Protein Accession #: NP_067258.1
• Nucleotide Accession #: NM_021283.2