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Catalog No: OKGD00017
Size:96 Wells
Price: $439.00
SKU
OKGD00017
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IL-4 ELISA Kit (Human) (OKGD00017)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 2 hours
ELISA Kit PrincipleAviva Systems Biology’s IL-4 ELISA Kit (Human) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL-4 has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A Biotin conjugated detector antibody specific for IL-4 is added, incubated and followed by wash. Next it is incubated with horseradish peroxidase (HRP)-Streptavidin ,followed by wash. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL-4 captured in well.
ELISA Kit Range3.13-200pg/ml
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 10%
Mean Inter-assay CV%: <= 10%
ELISA Kit Component
ComponentQuantity
Anti-IL-4 Microplate1 x 96 wells plate
IL-4 Lyophilized Standard2 x vials (200pg/ml/vial)
Human IL-4 Biotin Detection Antibody1 x 30 uL
Assay Diluent A1 x 50 mL
HRP-Streptavidin1 x 30 uL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Reconstitution and StorageStore as indicated in product manual.
Sensitivity<0.5pg/ml
Concentration200pg/ml
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL-4
Gene Full Nameinterleukin 4
Alias SymbolsBSF1, IL-4, BCGF1, BSF-1, BCGF-1
NCBI Gene Id3565
Protein NameInterleukin-4
Description of TargetHuman IL-4 participates in at least several B-cell activation processes as well as of other cell types . It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages . Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Uniprot IDP05112
Protein Accession #NP_000580.1, NP_758858.1
Nucleotide Accession #NM_000589.3, NM_172348.2
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