IL 27 Protein (OOPA00059)

Catalog No: OOPA00059 (Formerly GWB-BSP056)

Size:1UG

Product Info

• Product Format: Sterile Filtered White lyophilized (freeze-dried) powder. The IL-27 was lyophilized from 1mg/ml in 1xPBS.
• Reconstitution and Storage: Reconstitution: Centrifuge vial before opening. It is recommended to reconstitute the lyophilized IL-27 in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
  Storage: Lyophilized IL-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL27 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  
• Source: HEK.
• Purity: Greater than 90% as observed by SDS-PAGE.
• Biological Activity: The specific activity was determined by the dose dependent inhibition of HIV replication in human CD4+ T cells and in human monocyte-derived macrophages and is typically 4-12ng/ml.
• Peptide Sequence: IL-27A (215aa):
  FPRPPGRPQL SLQELRREFT VSLHLARKLL AEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP.
  IL-27B (209aa):
  RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK.
• Purification: The IL-27 is purified by proprietary chromatographic techniques.

Target Info

• Gene Symbol: IL 27
• Alias Symbols: Interleukin-30, IL-30, IL27/p28, p28, Interleukin-27, Interleukin-27/p28, IL27, Interleukin-27 subunit alpha, IL27 subunit alpha, IL27-A, Il27, Il27a, IL27p28.
• Description of Target: Interleukin-27 protein is related to IL-12A & is one of the subunits of a heterodimeric cytokine complex. IL27 interacts with EBV induced gene 3 also called EBI3, and forms a complex that drives rapid expansion of CD4 (+) T cells. IL27 complex is synergizes with IL-12 in order to trigger the cytokine production of IFN-Gamma of CD4 (+) T cells. The biological effect of IL27 is mediated by class-I cytokine receptor (WSX1/TCRR). The pro-inflammatory activity of IL27 is mediated through the growing expression of key molecules involved in the MHC class-I & MHC class-II pathways. Both MHC class-I and MHC-class-II expression are increased in endothelial cells after Interleukin-27 stimulation which suggests that it may play an important role in conferring the immune function on vascular endothelium IL27p28 subunit can be induced by IFN-beta and during LPS-induced maturation of dendritic cells in type-I IFN-dependent manner through IFN regulatory factor-1 activation. Interleukin-27 regulates Interleukin-12 responsiveness of CD4+ T cells through Stat1-dependent and -independent mechanisms. IL17 & IL-23 play an important IL17 role in inflammation. Interleukin-27 possesses potent anti-angiogenic activity that plays an important role in its antitumor and antimetastatic activities. EBV induced gene 3 plays a role, independently from IL27, in regulating anti-viral or anti-tumoral immune responses. Interleukin-27 is a potent inhibitor of HIV-1 replication in macrophages, CD4+ T cells, peripheral blood mononuclear cells. Interleukin-27 triggers STAT activation and gene transcription.
• Protein Size (# AA): Recombinant