Catalog No: OKBB00439
Size:96 Wells
Price: $583.00
SKU
OKBB00439
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse IL-27 p28 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-27 p28 has been precoated onto 96-well plates. Standards(NS0, F29-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-27 p28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL-27 p28 amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)103368556127407674
SD5.8725.7636.78.1332.9751.9
CV%5.776.66.48.17.7
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse IL-27 p28 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse IL-27 p28 Standard2 x 1 ng/tube
100X Biotinylated Anti-Mouse IL-27 p28 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse IL-27 p28
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: F29-S234
DescriptionFor quantitative detection of mouse IL-27 p28 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolIL27
Gene Full Nameinterleukin 27
Alias SymbolsIL 27A, IL 30, IL-27 subunit alpha, IL-27-A, IL27, IL27-A, IL27A, IL27A_HUMAN, IL27p28, IL30, Interleukin 27, Interleukin 27 subunit alpha, Interleukin 30, Interleukin-27 subunit alpha, p28
NCBI Gene Id246779
Protein NameInterleukin-27 subunit alpha
Description of TargetInterleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. By genomic sequence analysis, the IL27 p28 gene was mapped to chromosome 16p11. L-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130.
Uniprot IDQ8K3I6
Protein Accession #NP_663611.1
Nucleotide Accession #NM_145636.1
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