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Catalog No: OKAG00186 (Formerly GWB-SKR186)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00186
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Murine IL-27 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mIL-27 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IL-27 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range16-1000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (2 ng)
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity16 pg/mL
SpecificityThe Murine IL-27 ELISA is capable of recognizing both recombinant and naturally produced Murine IL-27 proteins. The antigens listed below were tested at 100 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IL-12 p40
• Murine: IL-6, IL-6R, IL-12, IL-12 p35, IL-12 p40, IL-23, IL-23 p19, TCCR/Fc Chimera
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolEbi3, Il27
Gene Full NameEpstein-Barr virus induced gene 3, interleukin 27
Alias SymbolsEBI-;EBI-3;epstein-Barr virus-induced gene 3 protein homolog;Il;IL-;IL-27;IL-27 p28 subunit;IL-27 subunit alpha;IL-27 subunit beta;IL27-A;IL-27-A;IL-27B;IL-27p28;Il30;interleukin 30;interleukin-27 subunit alpha;interleukin-27 subunit beta;p2;p28.
NCBI Gene Id246779, 50498
Protein NameO35228, Q8K3I6
Description of TargetInterleukin-27 subunit alpha, Interleukin-27 subunit beta
Uniprot IDhttps://www.uniprot.org/uniprot/?query=O35228+OR+Q8K3I6
Protein Accession #NP_663611.1
Nucleotide Accession #NM_145636.1
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