Catalog No: OKAG00144 (Formerly GWB-SKR144)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00144
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human IL-24 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-24concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope onHuman IL-24while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range63-4000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate12 x 8-WellMicrostrips
Protein StandardLyophilized (11.7ng)
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30 μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity63 pg/mL
SpecificityThe Human IL-24 ELISA is capable of recognizing both recombinant and naturally produced Human IL-24 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IL-20Rα, IL-20Rβ, IL-22R
• Murine: IL-24
Publications

Controlled Secretion of the Anticancer Protein MDA-7 from Engineered Mesenchymal Stem Cells. Biol. Pharm. Bull. 40, 113-117 (2017). 28049944

Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolIL24
Gene Full Nameinterleukin 24
Alias SymbolsC49A;FISP;IL10B;IL-4-induced secreted protein;interleukin-24;MDA7;melanocyte-associated Mda-7;melanoma differentiation-associated gene 7 protein;MOB5;ST16;suppression of tumorigenicity 16 (melanoma differentiation);Suppression of tumorigenicity 16 protein.
NCBI Gene Id11009
Protein NameQ13007
Description of TargetInterleukin-24
Uniprot IDhttps://www.uniprot.org/uniprot/Q13007
Protein Accession #NP_001172085.1
Nucleotide Accession #NM_001185156.1
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