IL-23 ELISA Kit (Human) : 96 Wells (OKAG00143)

Catalog No: OKAG00143 (Formerly GWB-SKR143)

Size:12x8WELLS

IL-23 ELISA Kit (Human) (OKAG00143) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Product Format: 1 x 96-Well Plate or 12 x 8-Well Strips
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric 450 nm
• ELISA Kit Duration: 4.5 hours
• ELISA Kit Principle: The Aviva Human IL-23ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIL-23 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human IL-23 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
• ELISA Kit Range: 63-8000 pg/ml
• ELISA Kit Component:
  • Capture Antibody Coated Microplate: 96 Wells (12 x 8)
  • Ready-to-Use Streptavidin-HRP: 12 mL
  • Ready-to-Use Substrate: 12 mL
  • Stop Solution: 12 mL
  • Wash Buffer (15x): 50 mL
  • Protein Standard Diluent: 12 mL
  • Sample Diluent: 12 mL
  • Detection Antibody Diluent: 12 mL
  • Biotin-Labeled Detection Antibody: Lyophilized
  • Protein Standard: Lyophilized (53 ng)
• Reconstitution and Storage: 2°C to 8°C
• Sample Type: Cell lysates, sera and plasma
• Sensitivity: 63 pg/mL
• Specificity: The Human IL-23 ELISA is capable of recognizing both recombinant and naturally produced Human IL-23 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
  • Human: IL-6, IL-11, IL-12, IL-12 p40, IL-12 Rβ2, IL-23 p19
  • Murine: IL-23, IL-23 p19
• Assay Info: Quantitative Colorimentric Sandwich ELISA

Target Info

• Gene Symbol: IL12B, IL23A
• Gene Full Name: interleukin 12B, interleukin 23 subunit alpha
• Alias Symbols: CLMF;CLMF p40;CLMF2;cytotoxic lymphocyte maturation factor 40 kDa subunit;IL-12 subunit p40;IL12, subunit p40;IL-12B;IL-23;IL-23 subunit alpha;IL-23A;IL-23-A;IL-23p19;IL23P19;IMD28;IMD29;interleukin 12, p40;interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40);interleukin 23 p19 subunit;interleukin 23, alpha subunit p19;interleukin-12 beta chain;interleukin-12 subunit beta;interleukin-23 subunit alpha;interleukin-23 subunit p19;interleukin-six, G-CSF related factor;JKA3 induced upon T-cell activation;natural killer cell stimulatory factor, 40 kD subunit;NK cell stimulatory factor chain 2;NKSF;NKSF2;P19;SGRF.
• NCBI Gene Id: 3593, 51561
• Protein Name: P29460, Q9NPF7
• Description of Target: Interleukin-12 subunit beta, Interleukin-23 subunit alpha
• Uniprot ID: https://www.uniprot.org/uniprot/?query=P29460+OR+Q9NPF7
• Protein Accession #: NP_057668.1
• Nucleotide Accession #: NM_016584.2