IL 21 Protein (OOPA00045)

Catalog No: OOPA00045 (Formerly GWB-BSP055)

Size:2UG

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: Escherichia Coli.
• Additional Information: Solubility: It is recommended to Reconstitution: Reconstitute the lyophilized Rat IL21 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
  Product Introduction: IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (C) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
  Product Description: Interleukin-21 Rat Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 129 amino acids and having a total molecular mass of 15.2kDa. The Rat IL-21 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Rat IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Rat IL21 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Formulation: Rat IL-21 was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
• Purification: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: The biological activity is measured by its ability to proliferate activated B cells.
• Peptide Sequence: HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS.

Target Info

• Gene Symbol: IL21
• Alias Symbols: Interleukin-21, IL-21, Il21.
• Protein Size (# AA): Recombinant