IL-2 High Sensitivity ELISA Kit (Human) (OKDB00044)

Catalog No: OKDB00044

Human IL-2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Linearity: Two pooled human sera, one human plasma and one cell culture medium samples containing different concentrations of IL-2 were serially diluted in standard buffer diluent. Linearity was evaluated on 4 dilutions. The linear regression of samples versus the expected concentration yielded a quote slope of 0.996.
• ELISA Kit Principle: Aviva Systems BiologyIL-2 High Sensitivity ELISA Kit (Human) (OKDB00041)is designed for the highly sensitive in-vitro quantitative measurement of recombinant or natural IL-2 in human serum, plasma or cell culture media. The kit isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaymethods along with an additional detection process employing a polymerization step to amplify the number of detector bound HRP molecules. A monoclonal antibody specific for IL-2 has been pre-coated into wells of a 96-wellplate. Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for IL-2 is added, incubated and followed by washing. Streptavidin-HRP conjugate is then added, incubated and unbound conjugate is washed away. Amplification is achieved by adding a Biotin-Tyramine reagent, incubating and washing, followed by a second incubation with Streptavidin-HRP conjugate. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL-2 captured in well.
• ELISA Kit Range: 1.87 - 60 pg/mL
• ELISA Kit Recovery: The recovery in pooled human sera ranged from 89 to 111% with an average of 100%. In cell culture medium, recovery was 95%.
• ELISA Kit Reproducibility: Four sera and one cell culture medium samples with various concentrations of IL-2 were tested for repeatability and reproducibility. Each assay was carried out with 3 replicates of each sample. Five independent assays were performed. The intra-assay and inter-assay coefficient of variation has been calculated to be 4.2% and 9.0% respectively.
• ELISA Kit Component:

  Reagents	1 x 48 Well Kit	1 x 96 Well Kit	2 x 96 Well Kit
  IL-2 Well Strip Microplate	6 x 8 Wells	12 x 8 Wells	24 x 8 Wells
  IFN gamma Standard	1 Vial	2 Vials	4 Vials
  10X Standard Diluent (Buffer)	1 x 25 mL	1 x 25 mL	1 x 25 mL
  27.5X Biotinylated anti-IFN gamma Detector Antibody	1 x 400 uL	1 x 400 uL	2 x 400 uL
  Biotinylated Detector Antibody Diluent	1 x 7 mL	1 x 7 mL	1 x 13 mL
  Streptavidin-HRP	1 x 5 uL	2 x 5 uL	4 x 5 uL
  Amplification Diluent	1 x 25 mL	1 x 25 mL	1 x 25 mL
  100X Amplifier	1 x 200 uL	1 x 200 uL	2 x 200 uL
  HRP Diluent	1 x 25 mL	1 x 25 mL	2 x 25 mL
  200X Wash Buffer	1 x 10 mL	1 x 10 mL	2 x 10 mL
  TMB Substrate	1 x 11 mL	1 x 11 mL	2 x 24 mL
  H2SO4 Stop Reagent	1 x 11 mL	1 x 11 mL	2 x 11 mL

• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, cell culture medium.
• Sensitivity: < 0.97 pg/mL
• Specificity: Ten specificities were tested with concentrations higher than IL-2 curve concentrations. No cross reaction was observed for concentrations ranging from 300 to 18.75 pg/ml for IL-1 alpha and beta, IL-8, IL-10, TNFalpha, GrB, FasL, TRAIL, CD116 and Gp-130..
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL-2
• Gene Full Name: interleukin 2
• Alias Symbols: IL-2, TCGF, lymphokine
• NCBI Gene Id: 3558
• Protein Name: interleukin-2 precursor
• Description of Target: The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
• Uniprot ID: P60568
• Protein Accession #: NP_000577.2
• Nucleotide Accession #: NM_000586.3
• Protein Size (# AA): 153
• Molecular Weight: 18 kDa