IL-1beta ELISA Kit (Mouse) (OKBB00178)

Catalog No: OKBB00178

Size:96 Tests

Mouse IL-1B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V118-S269) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL-1 beta amount of sample captured in plate.
• ELISA Kit Range: 12.5 pg/ml - 800 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	86	376	587	79	346	558
  St. Dev.	6.97	19.55	24.07	7.03	25.6	37.94
  %CV	8.10	5.19	4.10	8.89	7.39	6.79

• ELISA Kit Component:
  • Lyophilized recombinant mouse IL-1βstandard: 10 ng/tube x 2
  • Anti-mouse IL-1βAntibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-mouse IL-1βantibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 7.8pg/ml-500pg/ml
  Principle: Aviva’s mouse IL-1β ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL1-β specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1β amount of sample captured in plate. (The mouse IL-1βdetected in sera, plasma, body fluids, tissue lysates or cell culture supernates with this ELISA kit is mainly activated mature protein.)
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Interleukin-1β ( IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <1 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Takahashi, J. L.; Giuliani, F.; Power, C.; Imai, Y.; Yong, V. W. : Interleukin-1-beta promotes oligodendrocyte death
  through glutamate excitotoxicity. Ann. Neurol. 53: 588-595, 2003.
  2. Baek, S. H.; Ohgi, K. A.; Rose, D. W.; Koo, E. H.; Glass, C. K.; Rosenfeld, M. G. : Exchange of N-CoR corepressor
  and Tip60 coactivator complexes links gene expression by NF-kappa-B and beta-amyloid precursor protein. Cell 110:
  55-67, 2002.
  3. Voronov, E.; Shouval, D. S.; Krelin, Y.; Cagnano, E.; Benharroch, D.; Iwakura, Y.; Dinarello, C. A.; Apte, R. N. : IL-1 is
  required for tumor invasiveness and angiogenesis. Proc. Nat. Acad. Sci. 100: 2645-2650, 2003.
  4. Chen, C.-N.; Li, Y.-S. J.; Yeh, Y.-T.; Lee, P.-L.; Usami, S.; Chien, S.; Chiu, J.-J. : Synergistic roles of platelet-derived
  growth factor-BB and interleukin-1-beta in phenotypic modulation of human aortic smooth muscle cells. Proc. Nat.
  Acad. Sci. 103: 2665-2670, 2006.
• Specificity: Natural and recombinant mouse IL-1 beta
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: V118-S269
• Description: For quantitative detection of mouse IL-1 beta in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: IL1B
• Gene Full Name: interleukin 1, beta
• Alias Symbols: Catabolin, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL-1 beta, IL1, IL1 BETA, IL1B, IL1B_HUMAN, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, Interleukin-1 beta, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta
• NCBI Gene Id: 16176
• Protein Name: Interleukin-1 beta
• Description of Target: Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
• Uniprot ID: P10749
• Protein Accession #: NP_032387.1
• Nucleotide Accession #: NM_008361.4