IL 19 Protein (OPPA00672)

Catalog No: OPPA00672 (Formerly GWB-42D920)

Size:2UG

Product Info

• Predicted Species Reactivity: Horse
• Product Format: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Interleukin-19 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The biological activity was determined by measuring the dose-dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100ng/mL of IL-19 recombinant mouse.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard.
  Product Introduction: IL19 is a cytokine that belongs to the IL10 cytokine subfamily. IL-19 is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described.
  Product Description: Interleukin-19 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17722 Dalton. The IL-19 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Interleukin-19 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL19 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Met - Leu - Arg - Arg - Cys.

Target Info

• Gene Symbol: IL19
• Alias Symbols: Melanoma differentiation association like protein, MDA1, NG.1, ZMDA1, IL-10C, IL-19, IL 19 Mouse, Interleukin-19 Mouse Recombinant
• NCBI Gene Id: 329244
• Protein Name: Interleukin-19
• Description of Target: Recombinant Mouse Interleukin-19
• Uniprot ID: Q8CJ70
• Protein Accession #: NP_001009940.1
• Protein Size (# AA): Recombinant