Size:96 Wells
$419.00
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OKAG00028
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IL-17E ELISA Kit (Human) : 96 Wells (OKAG00028)

Catalog#: OKAG00028
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-SKR028
More Information
Predicted Species ReactivityHuman
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationELISA
Kit Detection MethodColorimetric, OD450 nm
Kit PrincipleThe Aviva Human IL-17E ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-17E concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-17E while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Range32-2,000 pg/mL
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Protein StandardLyophilized (83 ng)
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30 μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeCell lysates, sera and plasma
Sensitivity32 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityThe Human IL-17E ELISA is capable of recognizing both recombinant and naturally produced Human IL-17E proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Human: IL-17B
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IFN-γ, IL-8 (72 aa), IL-8 (77 aa), IL-10, IL-12, IL-12p40, IL-17A, IL-17D, IL-17F
• Murine: IL-17A, IL-17F
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolIL25
Official Gene Full Nameinterleukin 25
Alias SymbolsIL17E, IL-17E, IL-25, Interleukin-17E, Interleukin-25, UNQ3120/PRO10272
NCBI Gene Id64806
Protein NameInterleukin-25
Description of TargetThe protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.
Swissprot IdQ9H293
Protein Accession #NP_073626.1
Nucleotide Accession #NM_022789.3
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express IL25.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express IL25.
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