IL-17C ELISA Kit (Mouse) (OKBB00438)

Catalog No: OKBB00438

Size:96 Wells

Mouse IL-17C ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse IL-17C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17C has been precoated onto 96-well plates. Standards(E.coli, D17-Q194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL-17C amount of sample captured in plate.
• ELISA Kit Range: 125 pg/ml - 8,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.3	3.8	5.7	1.6	4.5	6.3
  SD	0.053	0.186	0.296	0.086	0.279	0.422
  CV%	4.1	4.9	5.2	5.4	6.2	6.7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse IL-17C Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse IL-17C Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse IL-17C Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse IL-17C
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: D17-Q194
• Description: For quantitative detection of mouse IL-17C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: IL17C
• Gene Full Name: interleukin 17C
• Alias Symbols: CX2, Cytokine CX2, IL 17C, IL 21, IL-17C, IL17C, IL17C_HUMAN, Interleukin 17C, Interleukin-17C, MGC126884, MGC138401
• NCBI Gene Id: 234836
• Protein Name: Interleukin-17C
• Description of Target: IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
• Uniprot ID: Q8K4C5
• Protein Accession #: NP_665833.3
• Nucleotide Accession #: NM_145834.3