Catalog No: OKDB00027
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OKDB00027
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IL-17A ELISpot Assay Kit (Human) (OKDB00027)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva's Human IL-17A ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope
ELISA Kit ComponentReagents sufficient for 96 Wells:
1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells)
2. Biotinylated detection antibody
3. Streptavidin-Alkaline Phosphatase conjugate
4. Bovine Serum Albumin
5. Ready to use BCIP/NBT substrate buffer
Reconstitution and Storage2 to 8C, Do not use past expiration date.
Sample TypeCell Suspensions
Assay InfoAssay Methodology: Quantitative Sandwich ELISpot Immunoassay
Gene SymbolIL-17A
Gene Full Nameinterleukin 17A
Alias SymbolsIL17, CTLA8, IL-17, CTLA-8, IL-17A
NCBI Gene Id3605
Protein Nameinterleukin-17A
Description of TargetThe protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Uniprot IDQ16552
Protein Accession #NP_002181.1
Nucleotide Accession #NM_002190.2
Protein Size (# AA)155
Molecular Weight18 kDa
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