Catalog No: OKAG00027 (Formerly GWB-SKR027)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00027
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human IL-17A ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIL-17A concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human IL-17A cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range8-2000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity8 pg/mL
SpecificityThe Human IL-17A ELISA is capable of recognizing both recombinant and naturally produced Human IL-17A proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IFN-γ, IL-8 (72 aa), IL-8 (77 aa), IL-10, IL-12, IL-12p40, IL-16 (121 aa), IL-16 (130 aa), IL-17B, IL-17D, IL-17E, IL-17F
• Murine: IL-17A, IL-17F
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolIL17A
Gene Full Nameinterleukin 17A
Alias SymbolsCTLA8;CTLA-8;cytotoxic T-lymphocyte-associated antigen 8;cytotoxic T-lymphocyte-associated protein 8;IL17;IL-17;IL-17A;ILA17;interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8);interleukin-17;interleukin-17A.
NCBI Gene Id3605
Protein NameQ16552
Description of TargetInterleukin-17A
Uniprot IDhttps://www.uniprot.org/uniprot/Q16552
Protein Accession #NP_002181.1
Nucleotide Accession #NM_002190.2
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