IL 15 Protein (OPPA00607)

Catalog No: OPPA00607 (Formerly GWB-BA53E1)

Size:2ug

Product Info

• Product Format: The protein was lyophilized from a concentrated (1mg/ml) solution with 10mM NaP pH 8.0. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be 15 ng/ml, corresponding to a Specific Activity of 2 x 10⁵ IU/mg.
  Product Introduction: The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.
  Product Description: Interleukin-15 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13321 Dalton. The IL-15 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL15 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Met - Asn - Trp - Ile - Asp.

Target Info

• Gene Symbol: IL15
• Alias Symbols: IL-15, AI503618
• NCBI Gene Id: 16168
• Protein Name: Interleukin-15
• Description of Target: Recombinant Mouse Interleukin-15
• Uniprot ID: P48346
• Protein Accession #: NP_032383.1