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Catalog No: OPPA00652 (Formerly GWB-42C802)
Size:2ug
Price: $103.00
SKU
OPPA00652
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IL 12 Protein (OPPA00652)

Datasheets/ManualsPrintable datasheet for OPPA00652
Product Info
Product FormatThe protein (1mg/ml) was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
HostBaculovirus
Additional InformationSolubility: It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
::Biological Activity: ED50 range = 0.01-0.05 ng/mL as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated mouse splenocytes that have been costimulated with PMA. Optimal concentration for individual application should be determined by a dose response assay.
::Product Introduction: IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.
Product Description: Interleukin-12 Rat Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques.
Reconstitution and StorageLyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL12 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
PurityGreater than 95.0% as determined by SDS-PAGE.
Peptide SequenceThe sequence of the first five N - terminal amino acids was determined and found to be Arg - Val - Ile - Pro - Val at the p35 subunit and Met - Thr - Glu - Leu - Glu at the p40 subunit.
Gene SymbolIL12
Alias SymbolsNKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12, IL 12 Rat, Interleukin-12 Rat Recombinant
Description of TargetRecombinant Rat Interleukin-12
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