Catalog No: OPPA00652 (Formerly GWB-42C802)
Size:2ug
Price: $103.00
SKU
OPPA00652
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA00652 |
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Product Format | The protein (1mg/ml) was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
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Host | Baculovirus |
Additional Information | Solubility: It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Biological Activity: ED50 range = 0.01-0.05 ng/mL as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated mouse splenocytes that have been costimulated with PMA. Optimal concentration for individual application should be determined by a dose response assay. |
:: | Product Introduction: IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity. Product Description: Interleukin-12 Rat Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL12 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and found to be Arg - Val - Ile - Pro - Val at the p35 subunit and Met - Thr - Glu - Leu - Glu at the p40 subunit. |
Gene Symbol | IL12 |
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Alias Symbols | NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12, IL 12 Rat, Interleukin-12 Rat Recombinant |
Description of Target | Recombinant Rat Interleukin-12 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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