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Catalog No: OKDB00007
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OKDB00007
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IL-10 ELISpot Assay Kit (Human) (OKDB00007)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva's Human IL-10 ELISpot Kit is based on standard sandwich ELISA methodology. Capture antibodies highly specific for the analyte of interest are coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and finally washed before adding the cells to be investigated. Cell suspension and stimulant are added to the coated and blocked microtitre plate and the plate incubated allowing the specific antibodies to bind any analytes produced. Biotinylated detection antibodies are then added which bind to the previously captured analyte. Enzyme conjugated streptavidin is added binding to the detection antibodies. Any excess unbound analyte and antibodies are removed by careful washing. Colour substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using microscopes.
ELISA Kit ComponentReagents sufficient for 96 Wells:
1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells)
2. Biotinylated detection antibody
3. Streptavidin-Alkaline Phosphatase conjugate
4. Bovine Serum Albumin
5. Ready to use BCIP/NBT substrate buffer
Reconstitution and Storage2 to 8C, Do not use past expiration date.
Sample TypeCell Suspensions
Assay InfoAssay Methodology: Quantitative Sandwich ELISpot Immunoassay
Gene SymbolIL-10
Gene Full Nameinterleukin 10
Alias SymbolsCSIF, TGIF, GVHDS, IL-10, IL10A
NCBI Gene Id3586
Protein Nameinterleukin-10
Description of TargetThe protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Uniprot IDP22301
Protein Accession #NP_000563.1
Nucleotide Accession #NM_000572.2
Protein Size (# AA)178
Molecular Weight21 kDa
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