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Datasheets/Manuals | Printable datasheet for IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Modification Sites: Human:S180/S181 Mouse:S180/S181 Rat:S180/S181 |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho |
Specificity | IKK-alpha/beta (Phospho-Ser180/181) Antibody detects endogenous levels of IKK-alpha/beta only when phosphorylated at Ser180/181. |
Application Info | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 Optimal dilutions should be determined by the end user. |
Gene Symbol | CHUK|IKBKB |
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Gene Full Name | component of inhibitor of nuclear factor kappa B kinase complex|inhibitor of nuclear factor kappa B kinase subunit beta |
Alias Symbols | BPS2;conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;I-kappa-B kinase 2;I-kappa-B kinase-alpha;I-kappa-B-kinase beta;IkB kinase alpha subunit;IKBKA;IKK1;IKK2;IKKA;IKK-a kinase;IKK-alpha;IKKB;IKK-beta;IMD15;IMD15A;IMD15B;inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta;inhibitor of nuclear factor kappa-B kinase subunit alpha;inhibitor of nuclear factor kappa-B kinase subunit beta;NFKBIKA;NFKBIKB;Nuclear factor NFkappaB inhibitor kinase alpha;Nuclear factor NF-kappa-B inhibitor kinase alpha;nuclear factor NF-kappa-B inhibitor kinase beta;serine/threonine protein kinase IKBKB;TCF16;TCF-16;transcription factor 16. |
NCBI Gene Id | 1147|3551 |
Protein Name | Inhibitor of nuclear factor kappa-B kinase subunit alpha|Inhibitor of nuclear factor kappa-B kinase subunit beta |
Description of Target | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.|Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor (PubMed:15084260). |
Uniprot ID | O14920|O15111 |
Molecular Weight | 84 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)"?
This target may also be called "BPS2;conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;I-kappa-B kinase 2;I-kappa-B kinase-alpha;I-kappa-B-kinase beta;IkB kinase alpha subunit;IKBKA;IKK1;IKK2;IKKA;IKK-a kinase;IKK-alpha;IKKB;IKK-beta;IMD15;IMD15A;IMD15B;inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta;inhibitor of nuclear factor kappa-B kinase subunit alpha;inhibitor of nuclear factor kappa-B kinase subunit beta;NFKBIKA;NFKBIKB;Nuclear factor NFkappaB inhibitor kinase alpha;Nuclear factor NF-kappa-B inhibitor kinase alpha;nuclear factor NF-kappa-B inhibitor kinase beta;serine/threonine protein kinase IKBKB;TCF16;TCF-16;transcription factor 16." in publications.
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What is the shipping cost for "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "84 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "IKK-alpha/beta Antibody (Phospho-Ser180/181) (OAAF07358)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CHUK|IKBKB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CHUK|IKBKB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CHUK|IKBKB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CHUK|IKBKB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CHUK|IKBKB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CHUK|IKBKB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.