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Catalog No: OKEH04742
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology IKBKB ELISA Kit (Human) (OKEH04742) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IKBKB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IKBKB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IKBKB captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 6.2%
Mean Inter-Assay CV: < 8.9%
ELISA Kit Component
IKBKB Microplate96 Wells (12 x 8 Well Strips)
IKBKB Standard2
Sample Diluent1 x 20 mL
IKBKB Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.79 ng/mL
SpecificityNatural and recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIKBKB
Gene Full Nameinhibitor of nuclear factor kappa B kinase subunit beta
Alias SymbolsI-kappa-B kinase 2;I-kappa-B-kinase beta;IKK2;IKKB;IKK-beta;IMD15;IMD15A;IMD15B;inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta;inhibitor of nuclear factor kappa-B kinase subunit beta;NFKBIKB;nuclear factor NF-kappa-B inhibitor kinase beta;serine/threonine protein kinase IKBKB.
NCBI Gene Id3551
Protein NameO14920
Description of TargetInhibitor of nuclear factor kappa-B kinase subunit beta
Uniprot ID
Protein Accession #NP_001177649.1
Nucleotide Accession #NM_001190720.2
Protein Size (# AA)756
Molecular Weight87 kDa
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