IHH ELISA Kit (Mouse) (OKCA02175)

Catalog No: OKCA02175

Size:96 Wells

Mouse IHH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	86	82-91
  Serum (n=4)	1:2	101	98-105
  Serum (n=4)	1:4	87	84-91
  Serum (n=4)	1:8	100	95-105

• ELISA Kit Principle: Aviva Systems Biology Ihh ELISA Kit (Mouse) (OKCA02175) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IHH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IHH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IHH captured in well.
• ELISA Kit Range: 3.12 pg/mL-200 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	89	85-94
  EDTA plasma (n=4)	107	103-110

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-IHH Microplate: 96 Wells (12 x 8 Well strips)
  • IHH Lyophilized Standard: 2 x 200 pg
  • 100X Biotinylated IHH Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.78 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IHH
• Alias Symbols: HHG-2
• NCBI Gene Id: 16147
• Protein Name: Indian hedgehog protein
• Description of Target: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
• Uniprot ID: P97812
• Protein Accession #: NP_034674
• Nucleotide Accession #: NM_010544