Catalog No: OPRA03654
Size:1MG
Price: $0.00
SKU
OPRA03654
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for IgM whole molecule Protein (OPRA03654) |
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Predicted Species Reactivity | Mouse |
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Product Format | Liquid (sterile filtered) |
Host | Mouse |
Conjugation | Unconjugated |
Application | ELISA, IF, WB |
Additional Information | Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 |
:: | Label: Unconjugated Preservative: 0.1% (w/v) Sodium Azide Stabilizer: None |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Purification | Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (μ chain specific). No reaction was observed against anti-Mouse IgG F(c). Some light chain cross-reactivity will occur with anti-Mouse IgG. |
Concentration | 1 mg/ml |
Application Info | Mouse IgM whole molecule can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments. All assays should be optimized by the user. |
Description of Target | Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is the primary antibody against A and B antigens on red blood cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen. |
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Protein Size (# AA) | Native |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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