Catalog No: OKEH03648
Size:96T
Price: $501.00
SKU
OKEH03648
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IGM ELISA Kit (Mouse) (OKEH03648) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IgM has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for IgM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgM captured in well.
ELISA Kit Range7.8-500ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.8% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
IgM Microplate96 Wells (12 x 8 Well strips)
IgM Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated IgM Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity3.91 ng/mL
SpecificityNatural and recombinant Mouse Immunoglobulin M
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIGM
Gene Full Nameimmunoglobulin heavy constant mu
Alias SymbolsIg, Igm, muH, Igh-, Igh6, Igh-6, Igh-M, AI326478
NCBI Gene Id16019
Protein Nameimmunoglobulin heavy constant mu
Description of TargetIgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions.
Uniprot IDP01872
Protein Size (# AA)475
Molecular Weight52 kDa
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