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Catalog No: OKGD00010
Size:96 Wells
Price: $439.00
SKU
OKGD00010
Availability: Domestic: within 1-2 days delivery | International: 1-2 days
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~2.5 hours
ELISA Kit PrincipleAviva Systems Biology IGM ELISA Kit (Human) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGM has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for IGMis added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGM captured in well.
ELISA Kit Range3.9 - 1,000 ng/mL
ELISA Kit Component
ComponentQuantity
Anti-IgM Microplate1 x 96 wells plate
IgM Lyophilized Standard2 x vials (1000 ng/vial)
IgM HRP-Detection Antibody1 x 10 uL
Assay Diluent A1 x 30 mL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, and other biological fluids
Sensitivity<= 3.0 ng/mL
Specificity
SpeciesCross Reactivity (%)
Chicken0
Dog1
Mouse0.12
Pig0.75
Rat0.25
AntigenCross Reactivity (%)
AAT0
APOA10
A2M0
C30
Fibrinogen0
HP0
HDL0
H. HSA0
IgA0
IgG0
LDL0
Orosomucoid0
Transferrin0
Gene SymbolIGM
Gene Full NameImmunoglobulin heavy constant mu
Alias SymbolsMU, VH, AGM1
NCBI Gene Id3507
Protein NameImmunoglobulin heavy constant mu
Description of TargetImmunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).
Uniprot IDP01871
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