IgM ELISA Kit (Human) (OKIA00215)

Catalog No: OKIA00215

Size:1 x 96 well plate

Representative Human IgM Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Human
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 100 Minutes
• ELISA Kit Principle: The Aviva Systems Biology IgM ELISA Kit (Human) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Immunoglobulin M (whole molecule) in Plasma or Serum. This assay employs an antibody specific for Human IgM coated on a 96-well plate. Standards and samples are pipetted into the wells and Immunoglobulin M (whole molecule) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-IgM antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 7.81 - 500 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Human IgM Microplate: 96 Wells (12 x 8 Well Strips)
  • Human IgM Calibrator: 1
  • 100X IgM-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The IgM test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM/Immunoglobulin M in Human biological samples.
  Sample Volume: 5 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 2.8 ng/mL

Target Info

• Alias Symbols: IgM;
• Protein Name: Immunoglobulin M (whole molecule)
• Description of Target: IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts (PubMed:3137579, PubMed:16895553).