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Catalog No: OKIA00196
Size:1 x 96 well plate
Price: $475.00
SKU
OKIA00196
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityFeline
Product Format12 of 8-well strips
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration70 Minutes
ELISA Kit PrincipleThe Aviva Systems Biology IgM ELISA Kit (Feline) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline Immunoglobulin M (whole molecule) in Plasma or Serum. This assay employs an antibody specific for Feline IgM coated on a 96-well plate. Standards and samples are pipetted into the wells and Immunoglobulin M (whole molecule) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-IgM antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Range6.25 - 400 ng/mL
ELISA Kit Reproducibility< 10%
ELISA Kit Component
ComponentAmount
Anti-Feline IgM Microplate96 Wells (12 x 8 Well Strips)
Feline IgM Calibrator1
100X IgM-HRP Conjugate150 uL
5X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Additional InformationIntended Use: The IgM test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM/Immunoglobulin M in Feline biological samples.
::Sample Volume: 5 uL
Reconstitution and StorageStore at 4 degrees
Sensitivity2.7 ng/mL
Alias SymbolsIgM
Protein NameImmunoglobulin M (whole molecule)
Description of TargetIgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts (PubMed:3137579, PubMed:16895553).
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