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IgM Antibody (OARA05275)

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Description of Target:
Anti-Chicken IgM antibody specifically detects chicken IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
Chicken Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Chicken IgM whole molecule
Product Format:
Liquid 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide
Reconstitution and Storage:
Store vial at 4°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid.   Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
1 mg/ml (UV absorbance at 280 nm)
Printable datasheet for OARA05275
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other Chicken heavy or light chain proteins.
Application Info:
Anti-Chicken IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA: 1:20000~100000
IHC: 1:1000~5000
WB: 1:2000~10000

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