Goat Anti-Chicken IgM (mu chain) Polyclonal Antibody (OARA05275)

Catalog No: OARA05275

Size:1MG

Product Info

• Predicted Species Reactivity: Chicken
• Product Format: Liquid (sterile filtered)
• Clonality: Polyclonal
• Host: Goat
• Conjugation: Unconjugated
• Application: Cell based assay|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid.   Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
• Immunogen: Chicken IgM whole molecule
• Purification: Chicken Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Concentration: 1.19 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other Chicken heavy or light chain proteins.
• Application Info: Anti-Chicken IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
  ELISA: 1:20000~100000
  IHC: 1:1000~5000
  WB: 1:2000~10000

Target Info

• Description of Target: Anti-Chicken IgM antibody specifically detects chicken IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.