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IgM Antibody (OARA05214)

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Description of Target:
Anti-Human IgM mu heavy chain antibody generated in goat detects specifically Human IgM mu heavy chain. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Anti-Human IgM mu heavy chain was produced by repeated immunization with Human IgM mu heavy chain in goat. Immunogen Type: Native Protein
Product Format:
Liquid. 0.02 M Postassium Phosphate, 0.15 M Sodium Chloride (pH 7.2) with 0.01% (w/v) Sodium azide.
Reconstitution and Storage:
Store vial at 4°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
1.0 mg/ml (by UV absorbance at 280 nm)
Printable datasheet for OARA05214
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Application Info:
ELISA: 1:10000~50000
IHC: 1:1000~5000
WB: 1:1000~5000
All assays should be optimized by the user.
Intended Use:
For research use only.

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