Catalog No: OKEH01967
Size:96T
Price: $600.00
SKU
OKEH01967
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IGIP ELISA Kit (Human) (OKEH01967) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human IGIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IGIP and incubated. The IGIP found in the sample or standards competes with the biotinylated IGIP for limited binding sites on the immobilized anti-human IGIP antibody. Excess unbound biotinylated IGIP and sample or standard IGIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IGIP captured in the well and inversely proportional to the amount of IGIP which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.1% (n = 20)
Mean Inter-assay CV%: < 10.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-IGIP Microplate96 Wells (12 x 8 Well strips)
IGIP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X IGIP-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human IgA-inducing protein homolog
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolIGIP
Gene Full NameIgA inducing protein
Alias SymbolsC5orf53;IgA-inducing protein homolog;IgA-inducing protein homolog (Bos taurus).
NCBI Gene Id492311
Protein NameA6NJ69
Description of TargetIgA-inducing protein homolog
Uniprot IDhttps://www.uniprot.org/uniprot/A6NJ69
Protein Accession #NP_001007190.1
Nucleotide Accession #NM_001007189.1
Protein Size (# AA)53
Molecular Weight5 kDa
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